Figure 2. Effects of irradiation on prostate cancer cells were enhanced by HULC knockdown. A dose of 6 Gy irradiation was delivered to cells. Expressions of cell cycle-associated proteins were down-regulated while expressions of apoptosis-associated proteins were up-regulated by HULC knockdown in PC3 cells (A) and LNCaP cells (B). C, Caspase-3 activity was enhanced by HULC knockdown. D, HULC silence arrested PC3 cells at G0/G1 phase. E, HULC overexpression arrested PC3 cells at S phase. Data are reported as means±SD. *P<0.05; **P<0.01 (t-test). HULC: long non-coding RNA highly upregulated in liver cancer; sh-HULC: pGPU6/GFP/Neo plasmid carrying short-hairpin RNA targeting HULC; sh-NC; pGPU6/GFP/Neo plasmid carrying a non-targeting RNA; pEX-HULC: pEX-2 plasmid carrying full-length HULC; PCNA: proliferating cell nuclear antigen.