Figure 3. Long-term effects of neonatal cigarette smoke exposure on brown adipose tissue (BAT) morphology. Representative hematoxylin and eosin staining of BAT at 180 postnatal days in males (A) and females (B) (Scale bar: 50 µm). Quantitative analysis of the sectional areas of BAT lipid vacuoles is shown in males (C) and females (D). C: control group; SE: smoke-exposed group. Data are reported as means±SE for n=6. There was gender effect, but there was no group effect for the lipid droplet sectional area (two-way ANOVA).