Figure 6. Long-term effects of neonatal cigarette smoke exposure on POMC and MC4R in the hypothalamus. Qualitative data of POMC (in green), counterstained for DAPI (in blue) in arcuate nucleus by immunohistochemistry (A) (Scale bar: 50 µm) and quantitative data concerning the number of POMC-positive cells in males (B) and females (C). Protein content of MC4R in the paraventricular nucleus (PVN) at 180 postnatal days in males (D) and females (E) with representative blots of proteins. β-Actin content was used as loading control. Data are reported as relative % to the control group. C: control group; SE: smoke-exposed group. Data are reported as means±SE for n=7 (two-way ANOVA re-examined by one-way ANOVA followed by Newman-Keuls post-hoc test).