Table 1. The nucleotide bias at each position of the T7 promoter library.
Position | Original base | G (%) | A (%) | T (%) | C (%) |
---|---|---|---|---|---|
-17 | T | 9.5 | 9.6 | 73.6 | 7.3 |
-16 | A | 11.5 | 66.6 | 12.9 | 9.0 |
-15 | A | 11.2 | 66.9 | 13.7 | 8.2 |
-14 | T | 9.5 | 9.0 | 73.8 | 7.7 |
-13 | A | 10.3 | 67.3 | 12.9 | 9.5 |
-12 | C | 13.2 | 11.7 | 13.7 | 61.3 |
-11 | G | 69.9 | 9.8 | 12.4 | 7.9 |
-10 | A | 11.0 | 66.1 | 13.1 | 9.8 |
-9 | C | 12.2 | 11.9 | 15.6 | 60.3 |
-8 | T | 9.6 | 8.9 | 73.9 | 7.6 |
-7 | C | 11.9 | 12.2 | 14.3 | 61.6 |
-6 | A | 10.7 | 66.8 | 13.3 | 9.2 |
-5 | C | 11.8 | 12.2 | 15.8 | 60.3 |
-4 | T | 10.1 | 10.4 | 72.3 | 7.2 |
-3 | A | 11.2 | 65.7 | 14.8 | 8.2 |
-2 | T | 10.1 | 11.0 | 72.3 | 6.6 |
-1 | A | 12.4 | 66.8 | 12.8 | 8.0 |
1 | G | 70.5 | 10.1 | 11.9 | 7.5 |
2 | G | 67.5 | 12.2 | 12.5 | 7.8 |