Table 1. PCR primers, probes and cycling conditions.

Gene	Primer Sequence	Probe	Amplicon	MgCl2, mM	Anneling T °C	Melting Temp (°C)
MMP2	for 5'-ataacctggatgccgtcgt-3' rev 5'-aggcacccttgaagaagtagc-3'	UPL #70	63 nt	2	60	95
MMP9	for 5'-atccggcacctctatggtc-3' rev 5'-ctgaggggtggacagtgg-3'	UPL #43,#6	121 nt	2	60	95
S1P1	for 5'-ggctggaactgcatcagtgcg-3' rev 5′-gagcagcgccacattctcagagc-3′		223 bp	4	60	89–90
S1P2	for 5′-ccgaaacagcaagttccact-3′ rev 5′-ccaggaggctgaagacagag-3′		197 bp	2	61	90
S1P3	for 5′-aaggctcagtggttcatcgt-3′ rev 5′-gctattgttgctgctgcttg-3′		201bp	2	61	92–93
S1P4	for 5′-ccttcagcctgctcttcact-3′ rev 5′-aagaggatgtagcgcttgga-3′		223 bp	4	64	94
S1P5	for 5′-aggacttcgcttttgctctg-3′ rev 5′-tctagaatccacggggtctg-3′		201bp	3	59	87
PBGD	for 5′-tccaagcggagccatgtctg-3′ rev 5′-agaatcttgtcccctgtggtgga-3′		204 bp	3	62	89
GAPDH	for 5'-gttcgacagtcagccgcatc-3' rev 5'-ggaatttgccatgggtgga-3'	5'- accaggcgcccaatacgaccaa -3'	229 nt	2	60	95
HPRT	for 5′-tgtaatgaccagtcaacaggg-3′ rev 5′-tggcttatatccaacacttcg-3′			2	60	94