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. 2018 May 7;13(5):e0197069. doi: 10.1371/journal.pone.0197069

Fig 3. DNA methylation status of the Peg3 domain among the four genotypes.

Fig 3

(A) The positions of three DMRs are indicated on the genomic map of the Peg3 domain. (B) The genotypes of the female and male sets of the pups are presented along with the results derived from the two sets of PCR amplifying the deleted regions. (C) The DNA from the four genotypes of both sexes were treated with bisulfite conversion protocol for DNA methylation analyses. The DNA were then amplified with four primer sets targeting the Peg3-DMR, the promoter of Zim1, Zim2-DMR and the Zfp264-DMR. The amplified products were digested with individual restriction enzymes that can differentiate the CpG methylation status of the original DNA, which are indicated with (U)nmethylation and (M)ethylation for a given CpG site.