Figure 4.

Increased BM thickness in Ossabaw pigs fed a Western diet. Representative EM images of retinal capillaries in the (A) superficial, (B) intermediate, and (C) deep capillary plexi, overlaid with a 20-spoke radial grid. Inset shows enlarged view of BM at spokes 20 and 10 for each image. BM was measured using ImageJ, by drawing a straight line perpendicular to the boundaries of the BM (denoted by arrowhead). Width measurements were averaged for the comparison between the lean and obese groups, which showed the BM to be consistently thicker across capillary plexi in the GCL, IPL, and INL/OPL of the obese Ossabaw pigs. n = 3 eyes per group; n ≥ 10 capillaries per eye. *P < 0.05 and ***P < 0.001. Scale bar: 1 μm.