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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Eur J Immunol. 2018 Jan 19;48(3):492–497. doi: 10.1002/eji.201747287

Figure 1. A–C: TLR ligand-stimulated autoantibody production.

Figure 1

(A) 1×106 B cells from Ig Tg mice were cultured with (+) or without (−) LPS stimulation. Anti-laminin Tg Ig is shown as mean +/− S.D. of OD405 on antigen-coated minus diluent-coated wells of duplicate samples, normalized to A10C antibody OD to control for interassay variability. *p<0.05 by nonparametric multiple comparison Wilcoxon Each Pair test, 4–5 mice per group, except unstimulated sample results pooled from all subjects (n=18). Ages in months as mean (range) are: Tg B6, 5.5 (3–8); Tg (B6xNZB)F1, 3.7 (3.5–4); Tg BWF1, 8.4 (6–10); and Tg NZB, 8.5 (8–9), from 1–2 experiments per strain. (B) Tg NZB (0.5×106 cultured cells, age 11–16 months from 2 experiments); Each line reflects paired samples from a single mouse +/− Mek1/2 inhibitor U0126; p=0.0625, Wilcoxon Signed Rank test; and, (C) Tg NZB (age 5–14 mon, 0.5×106 B cells per well), n=8–13 mice per group from 4 experiments, *p<0.05 vs each other group, Wilcoxon Each Pair test.