Fig. 4.

Enhancing GPP flux to the synthesis of CBGA enhances productivity of the strains. CBGA synthase is expressed on a high-copy plasmid under the control of a T5 promoter, whereas the rate-limiting enzymes of the non-mevalonate pathway, whose overexpression is critical for enhancing GPP production, are expressed on a medium-copy plasmid under the control of an IPTG-inducible Trc promoter. The replication and expression of both plasmids is compatible with one another. Error bars represent the standard deviation in the titer of CBGA