Fig. 2.

Cladribine induces marked memory B cells depletion. People with MS were treated with either subcutaneous cladribine of alemtuzumab and blood samples were taken at the last blood-screen prior to retreatment at year 1 (cladribine and alemtuzumab year 1) and 12 months after the second cycle (alemtuzumab year 2). Cells were stained with T and B cell markers assessed by flow cytometry and the results represent the mean ± standard deviation (n = 8/group). Flow cytometry of CD19 and CD27 staining in a a healthy control and b a person with MS treated with cladribine. c Total numbers of (left panel) T cells and (right panel) B cell subsets, d total numbers and e percentage of CD19⁺ B cells of immunoglobulin class-switched (IgD⁻) and unswitched (IgD⁺) and interleukin 2 receptor expressing memory B cells CD19⁺, CD27⁺ memory B cells