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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Biochemistry. 2017 Aug 1;56(32):4244–4255. doi: 10.1021/acs.biochem.7b00348

Figure 2. Absorption spectra of Cu(II)-peptides.

Figure 2

Absorption spectra for Hist-5 and model peptides (1 mM) with 0.8 equiv. of CuSO4 in 50 mM HEPES buffer, pH 7.4. The absorbance feature at 530 nm (ε = 110 M−1cm−1) is characteristic of a Cu(II)-bound ATCUN site. Replacement of the third His abolishes the ATCUN site, hence the absorbance red-shift to ~620 nm for peptide H3A.