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. Author manuscript; available in PMC: 2019 May 15.
Published in final edited form as: Bioorg Med Chem. 2018 Apr 6;26(9):2514–2529. doi: 10.1016/j.bmc.2018.04.016

Fig. 3.

Fig. 3

A: Adherent Ea.hy926 cells containing Fluo-4 dye. Cells were imaged in a clear-bottom, black wall 96-well plate, using an Evos Fl inverted microscope (20X) with GFP setting. B: Representative Fluo-4-Ca2+ emission curve (525 nm) in response to TFLLRN-NH2 (3.16 μM). C: Concentration–response curve of a typical (unsuccessful) assay with the PAR1 antagonist ML161 and 3.16 μM TFLLRN-NH2 before optimization (manual media exchanges), n = 3. D: Representative concentration–response curve with the PAR1 antagonist ML161 and 5 μM TFLLRN-NH2 after assay optimization (automated liquid handling).