Fig. 2. FAK is important for ovarian cancer cell invasion.

a Phase contrast images showing the morphology of stably transfected SKOV-3 cells with the indicated vectors followed by treatment with PF573228 (10 μM) for 4 days on Matrigel. Image original magnification ×100; scale bar, 200 μm. Inset shows higher magnification (left). The number of branches was counted and quantified (right); mean ± s.d. **P < 0.01 vs. the control vector, ^##P < 0.01 vs. RCP overexpression only. b SKOV-3 cells were transfected with the indicated vectors for 48 h followed by treatment with PF573228 (10 μM) for 1 h. Invasion assay (mean ± s.d. **P < 0.01 vs. the control vector, ^#P < 0.05 vs. RCP overexpression only). c SKOV-3 cells were co-transfected with the indicated vectors and siRNAs for 48 h prior to invasion assays (mean ± s.d. **P < 0.01 vs. the control vector, ^#P < 0.05 vs. RCP overexpression with scrambled siRNA). Original magnification, ×200; scale bar, 200 μm. All experiments were repeated three times