Fig. 3. UUO upregulated IL-33 in interstitial cells in obstructed kidneys.

a Immunofluorescence staining of IL-33 (red), vimentin (green), and nuclei with DAPI (blue) in kidney tissues from C57BL/6 mice with UUO surgery. b Expression of IL-33 in vimentin+ cells in the cortex, medulla, and ureter stromal cells. IL-33 was also detected in vimentin-negative multilayered urothelium cells in the UUO kidney. Yellow arrows indicate IL-33+vimentin+ cells. c Immunofluorescence staining of IL-33 (red), proximal tubules with Lotus lectin (LTL, green), and nuclei with DAPI (blue); *p < 0.05. d No co-localization of IL-33 with LTL+ cells was detected in the UUO kidney