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. 2018 Apr 20;50(4):36. doi: 10.1038/s12276-018-0047-8

Fig. 6. Deficiency of IL33 attenuated UUO-induced type 2 cytokine expression and loss of uroplakins.

Fig. 6

Total RNAs from the UPJ tissues of control and obstructed kidneys were collected from C57BL/6 (n = 5 from each group) and IL33KO mice (n = 5 from each group) for qRT-PCR analyses. a PC1 shows the log2 fold change in gene expression between UUO UPJ tissues and control (Ctrl) UPJ tissues from wild-type C57BL/6 mice. PC2 shows the log2 fold change in gene expression between IL33KO UUO UPJ tissues and C57BL/6 UUO UPJ tissues. The heatmap shows the gene expression of type 2 cytokines in UPJ tissues. Levels of b IL-13 and c IL-5 in control and hydronephrotic urine samples were analyzed using ELISA. d Heatmap of the gene expression of uroplakins in UPJ tissues. e Western blot analysis of the UPK3a protein in UPJ tissues. f Immunofluorescent staining of UPK3a and IL-33 in UPJ tissues