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. 2018 Apr 6;50(4):16. doi: 10.1038/s12276-018-0037-x

Fig. 4. Single-nucleotide substitution in zygotes with non-overlapping or overlapping sgRNAs into exon 2 of the mouse Adora2b locus.

Fig. 4

a A schematic diagram of the strategy for SNP induction (cA890G) with ssODN at the mouse Adora2b locus. The two different set of sgRNAs (non-overlapping; and overlapping, grey box) are shown. Blue letters indicate nucleotide alteration for amino-acid change and silent mutation for PCR-based genotyping. b PCR-based genotyping of pups and using specific primers. Red letter indicate KI pups. c Sequencing-based analysis for obtained pups. Green letter indicate A–G nucleotide substitution site (cA890G)