Fig. 3.
Clusterin (a) and progranulin production (b) by human airway epithelial cells after TDI exposure. Two HAECs, A549 cells and SAECs, were seeded (2 × 105 cells per mL) into 12-well plates and stimulated with TDI-HSA conjugate (2, 20, and 200 μg/mL) for 24 h. The clusterin and progranulin released from A549 cells and SAECs were measured by ELISA. Data are presented as the mean ± SEM of duplicate results from at least three independent experiments. *P < 0.05, **P < 0.001 obtained by one-way ANOVA with LSD post-hoc test. HAECs human airway epithelial cells, TDI-HSA TDI-human serum albumin conjugate, SAECs small airway epithelial cells