Fig. 5.
Effects of neutrophils on clusterin (a) and progranulin production (b) by human airway epithelial cells. Two HAECs (2 × 105 cells per mL)—A549 cells and SAECs—were co-cultured with different numbers of PBNs for 24 h. The ratios represent the number of epithelial cells to PBNs. The levels of clusterin and progranulin released into the culture supernatants were measured by ELISA. Data are presented as the mean ± SEM of duplicate results from at least three independent experiments. *P < 0.05, **P < 0.001 obtained by one-way ANOVA with LSD post-hoc test. HAECs human airway epithelial cells, PBNs peripheral blood neutrophils, SAECs small airway epithelial cells