Fig. 1. Elevated DANCR is expressed in colorectal cancer tissues and cell lines.

a, b DANCR endogenous expression levels were elevated in 91.49% (43/47) of CRC tissue specimens compared with those in matched non-CRC tissue specimens, as determined by qRT-PCR using GAPDH as the normalization control. **P < 0.01 vs. the non-CRC group. c The mean optical densities of DANCR were gradually elevated according to the grading of different clinical stages of CRC tissue specimens, as detected by in situ hybridization, normalized to the clinical stage I group. **P < 0.01, ***P < 0.001 vs. clinical stage I. d Endogenous expression of DANCR was up-regulated in the CRC cell lines HT29, HCT116, SW480, and LOVO compared with that in the normal human colon epithelial cell line NCM460, as measured by qRT-PCR using GAPDH as the normalization control. **P < 0.01, ***P < 0.001 vs. NCM460 group. The data are shown as the means ± SD from three independent experiments