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. 2018 May 1;50(5):57. doi: 10.1038/s12276-018-0082-5

Fig. 6. DANCR promotes proliferation and metastasis via the sponging of miR-577 in CRC cells.

Fig. 6

Fig. 6

a The wild-type DANCR over-expression plasmid pcDNA3.1-DANCR-wt, but not pcDNA3.1-DANCR-mut, could promote HSP27 expression in HT29 and LOVO CRC cells, and the facilitative effect was reversed by pre-miR-577, as detected by qRT-PCR. &P > 0.05, **P < 0.01 vs. pcDNA3.1 group; ##P < 0.01 vs. pcDNA3.1-DANCR-wt group. b The expression of LRP6 presented the same tendency as HSP27 after the same intervention, as detected by qRT-PCR. &P > 0.05, **P < 0.01 vs. pcDNA3.1 group; ##P < 0.01 vs. pcDNA3.1-DANCR-wt group. c DANCR negatively regulated miR-577 expression in HT29 and LOVO CRC cells, as detected by qRT-PCR. **P < 0.01 vs. the NC shRNA group, ##P < 0.01 vs. the pcDNA3.1-DANCR-wt group. d miR-577 also negatively regulated DANCR expression, as detected by qRT-PCR in HT29 and LOVO CRC cells. **P < 0.01 vs. the pre-miR-577-NC group, ##P < 0.01 vs. the anti-miR-577-NC group. e, f The wild-type DANCR over-expression plasmid pcDNA3.1-DANCR-wt, but not pcDNA3.1-DANCR-mut, promoted HT29 (e) and LOVO (f) cell proliferation, and the facilitative effect was reversed by pre-miR-577, as determined by the EDU (Scale bar, 25 µm; magnification, ×400) and CCK8 assays. &P > 0.05, **P < 0.01 vs. the pcDNA3.1 group; ##P < 0.01 vs. the pcDNA3.1-DANCR-wt group. g, h Wild-type DANCR over-expression plasmid pcDNA3.1-DANCR-wt, but not pcDNA3.1-DANCR-mut, promoted HT29 (g) and LOVO (h) cell metastasis, and the facilitative effect was reversed by pre-miR-577, as determined by Transwell assays. &P > 0.05, **P < 0.01 vs. the pcDNA3.1 group; ##P < 0.01 vs. the pcDNA3.1-DANCR-wt group (For E-H, Nos. 1–5 represent the pcDNA3.1 group, pcDNA3.1-DANCR-wt group, pcDNA3.1-DANCR-mut group, pcDNA3.1-DANCR-wt+pre-miR-577-NC group and pcDNA3.1-DANCR-wt+pre-miR-577 group, respectively). The data are shown as the means ± SD from three independent experiments