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. 2018 Apr 13;50(4):27. doi: 10.1038/s12276-018-0056-7

Fig. 5. Transcription factors responsible for circ-4099 expression.

Fig. 5

a Biotin-labeled circ-4099 promoter was mixed with nuclear extracts from hNP cells. b The eluted proteins in the red box were analyzed using mass spectrometry methods. c, d The GRP78/94 protein levels in the nuclei of hNP cells treated with TNF-α for 24 h were analyzed by WB. e, f The GRP78/94 protein levels in hNP cells transfected with shRNA vectors for 48 h were analyzed by WB. g circ-4099 expression was analyzed by qRT-PCR in hNP cells transfected with the sh-GRP78 RNA vector for 48 h and then treated with TNF-α for 24 h