Fig. 3. Morphology of ipo13-deficient zebrafish morphants.

a Lateral view of zebrafish larvae. Larvae injected with the highest does of ipo13 targeting MO at 5.0 ng/µL exhibit apparent spinal curvature and reduced eye size. Abnormalities were not observed in the low concentration groups. b Magnified lateral pictures of zebrafish eyeballs show significant decrease in eye size. c Vertical view of larval eyeballs displays shorter axial length in the highest MO dose group. d, e Quantification of the eyeball axial length (d) and eye area (e). The axial length and eye area are represented as a white line and red circle, respectively. Student’s t-test, values are means ± s.e.m. ***P < 0.001