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. 2018 Apr 20;50(4):41. doi: 10.1038/s12276-018-0062-9

Fig. 3. The Dll4/Notch1 pathway in the induced membrane in the subcutaneous site and femoral defect site after 6 weeks of surgery.

Fig. 3

a The mRNA expression levels of 9 ligands or receptors involved in Notch signaling (ligands: Dll1, Dll3, Dll4, Jagged1 and Jagged2; Receptors: Notch1, Notch2, Notch3 and Notch4) were screened by qRT-PCR in induced membrane, which formed in the subcutaneous site and femoral site at 2, 4, and 6 weeks (**P < 0.01, vs. 2-week induced membrane in the subcutaneous site and femoral defect site, respectively, ##P < 0.01, vs. the same time point for the induced membrane in the subcutaneous site). b–c The protein expression of Dll4, Notch1 (NICD), Hes1, VEGFR2, and CD31 in induced membrane derived from the subcutaneous site and femoral defect site. d–e Immunohistochemistry staining of Notch1 (NICD) in induced membrane derived from the subcutaneous site and femoral defect site (scale bar: 50 μm). The data in the figures represent averages ± S.D. Significant differences between the different groups are indicated as **P < 0.01, compared with induced membrane in the subcutaneous site; for each group, n = 5