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. 2018 Apr 13;50(4):21. doi: 10.1038/s12276-017-0002-0

Fig. 4. Commensal microbiota protect against severe colon cancer in SIRT3KO mice.

Fig. 4

a Images of distal colons from SIRT3KO and SIRT3KO+Abs mice after AOM/DSS tumorigenesis. b Comparison of polyp numbers between SIRT3KO and SIRT3KO+Abs mice (n = 5–6). The data are represented as the mean ± SEM. P < 0.05; **P < 0.01; ***P < 0.001; by unpaired Student’s t test. c Representative HE staining of distal colon sections obtained from SIRT3KO and SIRT3KO+Abs mice after AOM/DSS tumorigenesis. d Featured altered microbial communities between SIRT3KO and SIRT3KO+Abs mice after AOM/DSS tumorigenesis (n = 5–6) from Illumina Miseq sequencing. Data were analyzed by the Mann–Whitney test. e Fecal occult blood tests of SIRT3KO and SIRT3KO+Abs mice (n = 5–6). f PCoA of 16S rRNA gene-sequencing analysis of gut microbes obtained from the SIRT3KO and SIRT3KO+Abs mice (n = 5–6) from Illumina Miseq sequencing. PC1 and PC2 explain 51.02 and 14.66% of the variation, respectively. g Western blots of intestinal CLCN4, JNK, CLIC4, GSK3β and β-catenin expression from SIRT3KO and SIRT3KO+Abs mice after AOM/DSS tumorigenesis. h FISH with Lactobacillus (green) and Coriobacterium (red) from distal colons of SIRT3KO and WT mice after AOM/DSS tumorigenesis. n no significance