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. 2018 Apr 13;50(4):29. doi: 10.1038/s12276-018-0058-5

Fig. 5. Isolation and identification of exosomes from adipose-derived stem cell (ADSC) culture medium.

Fig. 5

a The ultrastructure of ADSC-derived exosomes by transmission electron microscopy. Scale bar, 100 nm. b Size distribution of Exo-ADSCs determined by dynamic light scattering. c Expression of the exosome markers CD4, CD63, and TSG101 and β-actin confirmed by immunoblotting. Exosome lysate was loaded into the left lane and cell lysate into the right. d Representative micrographs of EPCs or EPCs incubated with Dil-labeled Exo (+ExoDil) or with Dil-labeled EVs denatured by boiling (+dExo-Dil). Scale bar, 10 μm