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. 2018 Apr 27;50(4):45. doi: 10.1038/s12276-018-0068-3

Fig. 1. STAT3 is a direct regulatory target of miR-125a-5p in HEK-293T cells.

Fig. 1

a Schematic depiction of the pFLuc-3′-UTR-STAT3 luciferase reporter construct containing the 3′-UTR of the rat STAT3 gene; the predicted miR-125a-5p‒binding site (BS) is indicated. Shown below is the wild-type miRNA binding site (CUCAGGGA), along with the corresponding complementary sequence in the miR-125a-5p miRNA; the sequence of the mutated binding site (GAGTCCC) is also shown. b HEK-293T cells were transfected with the indicated constructs; two days after transfection, luciferase activity was measured and is expressed relative to cells transfected with the respective control miRNA. **p < 0.01; NS not significant (p > 0.05)