Fig. 2. Post-ischemic inflammation in MCAo rats treated with IV-hUMSCs.

a ED-1 and Iba-1 immunostaining of the peri-infarct area between the IV-hUMSC group and saline group (n = 5 per group). b iNOS and CD206 immunostaining of the peri-infarct area between the IV-hUMSC group and saline group (n = 5 per group). c Immunohistochemistry of neutrophil elastase (ELANE) in the peri-infarct area between the IV-hUMSC group and saline group (n = 5 per group). d Enzyme-linked immunosorbent assay (ELISA) of myeloperoxidase (MPO) in lysates of the ischemic hemisphere of rats with no MCAo (normal group, n = 5), rats with 24 h post MCAo (MCAo-24 h group, n = 5), rats with 72 h post MCAo treated with saline (saline group, n = 5), or 1 × 10⁶ IV-hUMSCs at 24 h post MCAo (IV-hUMSC group, n = 5). All data are presented as the mean ± SEM. *p < 0.05 or **p < 0.001. Scale bar = 20 μm