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. 2018 Apr 13;50(4):22. doi: 10.1038/s12276-018-0041-1

Fig. 5. In vitro expression of IL-1ra in LPS-stimulated macrophages after treatment with conditioned medium of hUMSCs.

Fig. 5

a IL-1β and IL-1ra ELISA of raw 264.7 cells exposed to three treatment conditions (no LPS treatment, LPS treatment, LPS, and hUMSCs-CM treatment). b Western blot analysis of CREB, p-CREB, NF-κB, and p-NF-κB in lysates of raw 264.7 cells exposed to three treatment conditions (no LPS treatment, LPS treatment, LPS, and hUMSCs-CM treatment). c IL-1ra ELISA after treatment with KG501 (2, 5, and 10 μM) in raw 264.7 cells exposed to three treatment conditions (no LPS treatment, LPS treatment, LPS, and hUMSCs-CM treatment). d Western blot analysis of IL-1ra after knockdown of CREB (siCREB) or control (siCtr) in raw 264.7 cells. The bands below the graph in the right panel show the gene expression of CREB and GAPDH by PCR. All data are presented as the mean ± SEM. *p < 0.05. LPS lipopolysaccharide, hUMSCs-CM conditioned medium of hUMSCs, siCREB siRNA for CREB, siCtr siRNA for control