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. Author manuscript; available in PMC: 2019 May 15.
Published in final edited form as: Anal Biochem. 2018 Mar 2;549:12–20. doi: 10.1016/j.ab.2018.03.003

Figure 2.

Figure 2

Figure 2

Figure 2

A. Oxidative phosphorylation: complexes I and II

B. Oxidative phosphorylation: complex IV

C. Oxidative phosphorylation: fatty acid oxidation and complex III

Mitochondrial OXPHOS flux of human mononuclear cells. Data are presented as mean ± standard error from 48 patients with prostate cancer without treatments. M: malate; P: pyruvate; ADP: adenosine diphosphate; G: glutamate; S: succinate; FCCP: carbonylcyanide-p-trifluoromethoxyphenylhydrazone; Rot: rotenone; AA: antimycin A; TMPD: tetramethyl-p-phenylenediamine; Pal: palmitoylcarnitine. OXPHOS complex I, complex II (Fig. 1A), and complex IV (Fig. 1B) determined with substrates uncoupler inhibitors titrations protocol 1; protocol 2 was used to determine fatty acid oxidation and complex III (Fig. 1C).