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. Author manuscript; available in PMC: 2019 May 15.
Published in final edited form as: Anal Biochem. 2018 Mar 2;549:12–20. doi: 10.1016/j.ab.2018.03.003

Table 3.

Integrated Mitochondrial Function in Human Peripheral Blood Mononuclear Cells (n=48)

Prorocol 1 (pmol/sec/million cells) mean ± standard error
Intact Cell (Malate+Pyruvate) 4.8 ± 0.2
Permeabilized Cell (Digitonin) 0.1 ± 0.1
OXPHOS (CI) 5.0 ± 0.4
OXPHOS (CI+CII) 14.2 ± 0.9
Maximal oxidative capacity (Uncoupler) 15.5 ± 0.9
Uncoupled CI 6.8 ± 0.5
Uncoupled CII 8.7 ± 0.6
Uncoupled CIV 227.3 ± 12.9
Protocol 2
Intact Cell (Malate+ Palmitoylcarnitine) 4.2 ± 0.2
Permeabilized Cell (Digitonin) 0.2 ± 0.1
OXPHOS (Fatty acid oxidation) 3.8 ± 0.3
OXPHOS (Coupled CIII; Duroquinol) 21.1 ± 1.1
Uncoupled Complex III (Uncoupler) 20.1 ± 1.2

OXPHOS=oxidative phosphorylation; CI=complex I; CII=complex II; CIII=complex III; CIV=complex IV. Data are subtracted from non-mitochondrial respiration (antimycin A respiration for protocol 1, rotenone and antimycin A respiration for protocol 2).