Table 4.
Comparison of ETC Enzymatic Assay in Mononuclear Cells and Lymphocytes (nmole/min/mg)
| References | Our data (N=46) | ||||
|---|---|---|---|---|---|
| Measures | Berger I. | Negari S.B. | Drouet M. | Rustin P. | |
| CS | NP | 75 | ND | 47–114 | 54±2.8 |
| SDH | ND | 15 | ND | ND | 4.1± 0.3 |
| I/III | ND | ND | ND | ND | 13.4±0.8 |
| II/III (Without antimycin A-sensitive) | ND | 20 | 24.3–32.9 | 23–46 | 11.2±0.7 |
| II/III (With antimycin A-sensitive) | 10.2±0.6 | ||||
| II−Q1 | ND | ND | ND | ND | 1.9±0.1 |
| II+Q1 | ND | ND | ND | ND | 9.2±0.5 |
| III | ND | ND | 44.2–51.5 | ND | 37.7±3 |
| IV | 0.75±0.12* | 80 | 49.4–50.1 | 69–146 | 1.2±0.1** |
| LDH | ND | ND | ND | 706–1656 | 533.8±30 |
ETC= electron transport chain; CS=citrate synthase; NP=Not provide; ND=Not determined; SDH=succinate dehydrogenase; I/III= rotenone-sensitive linked activity of complex I and III; II/III=antimycin Asensitive linked activity of complex II and III; II−Q1 and II+Q1=complex II activity as the thenoyltrifluoroacetone-sensitive without CoQ1 or with CoQ1; III= antimycin A-sensitive, complex III activity; IV= cytochrome c oxidase, complex IV activity ; LDH= lactate dehydrogenase;
*
= ratio of complex IV/CS;
**
= k−1/mg. A total of 46 samples were used for ETC analysis because the technician was on sick leave and not be able to perform 2 assays.