Table 1. The combination of blocking agents and antibodies used for immunohistochemistry.

Detection	Blocking reagent	Primary antibody	Secondary antibody
TH	Blocking reagent A and B	Mouse monoclonal antibody against TH	Histofine MAX-PO (M)
	(Nichirei Bioscience, Tokyo, Japan)	(MAB318; 1:5,000)	(Histofine Simplestain system)
		(Merck Millipore, Darmstadt, Germany)	(Nichirei Bioscience, Tokyo, Japan)
DRD1	Blocking reagent A and B	Mouse monoclonal antibody against DRD1	Histofine MAX-PO (M)
	(Nichirei Bioscience, Tokyo, Japan)	(MAB5290; 1:1,500)	(Histofine Simplestain system)
		(Merck Millipore, Darmstadt, Germany)	(Nichirei Bioscience, Tokyo, Japan)
DRD2	Blocking One Histo	Rabbit polyclonal antibody against DRD2	EnVision + System- HRP Labeled Polymer
	(Nacalai Tesque, Kyoto, Japan)	(AB5084P; 1:1,500)	Anti-Rabbit
		(Merck Millipore, Darmstadt, Germany)	(Dako, Glostrup, Denmark)

TH: Tyrosine hydroxylase, DRD₁: Dopamine receptor D₁, DRD₂: Dopamine receptor D₂.