Table 3. The results of virus isolation, RT-PCR analysis, immunohistochemistry (IHC) and histology from tissue samples (virus isolation/RT-PCR/IHC/histological lesion).
| Pig No. | 2 | 5 | 6 | 3 | 4 | 7 |
|---|---|---|---|---|---|---|
| dpi | 1 | 1 | 1 | 3 | 3 | 3 |
| Tongue | −/−/+/− | −/−/+/− | −/−/+/− | −/+/+/+ | 3.1/+/+/+ | 5.8/+/+/+ |
| Tonsil of the soft palate | −/+/+/− | −/−/+/− | 4.3/+/+/− | −/+/+/− | 6.3/+/+/− | 3.3/+/+/− |
| Soft palate | 2.8/+/−/− | −/+/−/− | −/−/−/− | −/+/−/− | 4.3/+/−/− | 3.3/+/−/− |
| Oropharynx | 2.6/+/−/− | 2.6/+/−/− | −/−/−/− | 2.6/+/−/− | 4.3/+/+/− | 2.6/+/+/− |
| Nasopharynx | −/−/−/− | −/+/−/− | −/−/−/− | −/−/−/− | 4.8/+/−/− | 2.8/+/−/− |
| Larynx | 3.1/+/−/− | −/+/−/− | −/−/−/− | −/−/−/− | 5.3/+/−/− | 2.8/+/−/− |
| Trachea | −/−/−/− | −/−/−/− | −/−/−/− | −/−/−/− | 4.1/+/−/− | 2.6/+/−/− |
| Lung | −/−/−/− | −/+/−/− | −/−/−/− | −/−/−/− | 3.8/+/−/− | 2.6/+/−/− |
| Esophagus | −/−/−/− | −/+/−/− | −/−/−/− | −/+/+/+ | 6.6/+/+/+ | −/+/+/+ |
| Stomach | −/−/−/− | −/−/−/− | −/−/−/− | −/+/−/− | 3.3/+/−/− | −/−/−/− |
| Ileum | −/−/−/− | −/−/−/− | −/−/−/− | −/−/−/− | 3.3/+/−/− | −/−/−/− |
| Rectum | −/−/−/− | −/−/−/− | −/−/−/− | −/−/−/− | 3.3/+/−/− | −/−/−/− |
| Heart | −/−/+/− | −/−/+/− | −/−/−/− | −/−/+/+ | 3.8/+/+/+ | −/+/+/+ |
| Spleen | −/−/−/− | −/−/−/− | −/−/−/− | −/−/−/− | 5.8/+/−/− | −/+/−/− |
| Thymus | −/−/−/− | −/−/−/− | −/−/−/− | −/−/−/− | 3.8/+/−/− | 2.6/+/−/− |
| Mandibular LN | −/−/−/− | −/−/−/− | −/−/−/− | −/−/−/− | 7.1/+/−/− | −/+/−/− |
| Parotid LN | −/−/−/− | −/−/−/− | −/−/−/− | −/−/−/− | 6.8/+/−/− | −/+/−/− |
| Retropharyngeal LN | −/−/−/− | −/−/−/− | −/−/−/− | −/−/−/− | 5.8/+/−/− | −/+/−/− |
| Cervical LN | −/−/−/− | −/−/−/− | −/−/−/− | −/−/−/− | 8.3/+/−/− | −/+/−/− |
| Mandibular gland | −/−/−/− | −/−/−/− | −/−/−/− | −/−/−/− | 3.8/+/−/− | 3.3/+/−/− |
| Parotid gland | −/−/−/− | −/−/−/− | −/−/−/− | −/−/−/− | 3.1/+/−/− | −/−/−/− |
| Skin of the snout | −/−/−/− | −/−/−/− | −/−/−/− | −/+/+/+ | 7.3/+/+/+ | −/−/+/+ |
| Skin of the coronet | −/−/+/+ | −/−/+/+ | −/−/+/+ | −/+/+/+ | 9.3/+/+/+ | 5.3/+/+/+ |
dpi: day(s) post inoculation; LN: lymph node; 3.1/+/+/+: 3.1 log10TCID50/ml/PCR positive/IHC positive/histological lesion positive. Samples showed virus isolation positive and RT-PCR positive are colored orange. Samples showed virus isolation negative but RT-PCR and IHC positive are colored yellow. Samples showed virus isolation negative but RT-PCR or IHC positive are colored light orange.