Table 2. Sequences of primers used in RT-qPCR for detection of S1 gene of IBV.

Region	Primer	Sequence	Positionb)	Reference
HVRI	C2	F-TGG TTG GCA TTT ACA CGG GG	20487–20506	[33]
	C3a)	R-CAA TGT GTA ACA AAC AC	20698–20714
HVRII	HV+	F-GTT ACA CAT TGT TAT AA	20704–20720	[27]
	HV−	R-ACA AGA TCA CCA TTT AA	20878–20894

F=Forward, R=Reverse. a) Modified, b) Annealing site on S1in M41 reference strain of IBV (sequence accession number: AY851295).