Table 3. Virus detection in pulmonary and tracheal samples from experimentally infected chickens (M41 or H120 strains) by RT-qPCR and virus isolation methods.

	Challenge	RT-qPCR	VI
Lung	M41	11/14	8/14
Trachea	M41	14/14	8/14
Lung	H120	1/3	1/3
Trachea	H120	3/3	1/3
Lung	Mock infected	0/14	0/14
Trachea	Mock infected	0/14	0/14

Birds of same group/interval (3 biological replicates) were pooled and processed as one sample by both RT-qPCR and VI.