Table 1. Quality metrics for this assembly compared to previous donkey genome assemblies.

The number of annotated genes (lower than that in previous assemblies) shows a better homologous correspondence with the horse gene set (see Gene annotation).

	This study	Huang et al. (15)	Orlando et al. (2)
N50 contigs	140.3 kb	66.7 kb	6.38 kb
N50 scaffolds	15.4 Mb	3.8 Mb	100.94 kb
Coverage	61.2×	42.4×	12.4×
Total bases	2.320 Gb	2.391 Gb	2.293 Gb
Largest scaffold	84.20 Mb	17.06 Mb	1.09 Mb
Unresolved bases per 100 kb	1121.61	1384.93	4128.43
Total number of predicted protein-coding genes	18,984	23,850*	24,156

*Calculated using one isoform per gene and 42,247 total transcripts.