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. 2018 May 8;4:22. doi: 10.1038/s41421-018-0018-1

Fig. 3. Recombinant mutant flagellin of Salmonella typhi (SF) without its C-terminal 35 amino acids fails to activate NLRC4 pathway.

Fig. 3

a Caspase-1 activation detected 1 h after transfection of 100 nM flagellins in LPS pretreated BMMs. b IL-1β secretion detected 20 h after transfection of 100 nM flagellins in LPS pretreated BMMs. c Co-immunoprecipitation assay of flagellin of E. coli K12 strain (KF), flagellin of Salmonella typhi (SF) and C35 deletion variant of SF with NAIP5 in 293T cells. d Co-immunoprecipitation assay of flagellins KF, SF, and C35 replacement variant of SF with NAIP5 in 293T cells. Shown are immunoblots of anti-Flag immunoprecipitates (IP: Flag) and total cell lysates (Input)