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. 2018 May 1;9:526. doi: 10.3389/fpls.2018.00526

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Copyright © 2018 Li, Li, Wu, Zhang, Zhu, Wang, Zeng, Xu, Fan, Sha, Zhang, Zhou and Kang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chromosome identification of hybrid progenies. Images on the right of each panel show FISH results using pSc119.2 (green), pTa535 (red), pAs1 (red), pTa713 (red), and pTa71 (green), and those on the left show corresponding GISH patterns. Chromosomes in blue and green are wheat and Th. elongatum chromosomes in the GISH patterns, respectively. (A,B) Line K16-712-1-2 had a complete set of 28 A/B chromosomes, 12 D (2D-6D) chromosomes, and a pair of 1E chromosomes. (C–E) Line K16-681-4 had 13 A (lacking one 3A), 16 B (four 7B), 13 D (lacking one 1D), and one pair of 2E chromosomes. (F–H) Line K16-562-3 had all 28 A/B chromosomes, 10 D (1D, 2D, 5D, 6D, and 7D) chromosomes, and a pair each of 3E and 4E chromosomes. (I–K) Line K17-1089-1 had a complete set of 42 A/B/D chromosomes and one 4E chromosomes.