Table 2.
Sequences of E-genome chromosomes specific markers.
| Marker | Primer sequence (5′–3′) | Annealing temperature (°C) | Amplified chromosomes | Reference |
|---|---|---|---|---|
| M1E_No.21 | F: CATCATATAAGAATGCAGGA R: TCAACAGCACGCCAAC | 55 | 1E | Dai et al., 2017b |
| SLAF 2E-22 | F: TTTTGATGGCGACTTTAGG R: CTTGGTTCCTGACCCTTT | 55 | 2E | Dai et al., 2017b |
| SLAF 3E-6 | F:GTCTACTTTAGCACCTCACTCA R: AAATTCGTCCGGTTGTT | 60 | 3E | Dai et al., 2017b |
| P4E-1 | F:TGTTGCCAGTGGAGGAAG R:TTGAACTACTGAACCGAAT | 55 | 4E | Chen et al., 2015 |
| P5E-1 | F:ACAGTGAGCCCGAAGGAA R:AACACCAGCGGACAAAGC | 60 | 5E | Chen et al., 2015 |
| SLAF 6E-15 | F: AGTAATTGGAGACCCAGGCG R: CAAATCAAACGACCATCACA | 60 | 6E | Dai et al., 2017b |
| M7E_No.53 | F:GTCAAGAGTTGGCTTTATTC R:ATTTGCTAATTCTCGTCATA | 60 | 7E | Chen et al., 2013 |