Figure 3.

Dimensions of the mother cell and its doubling times in dead-end channels of different widths. (A–D) Mother cell length, width, volume and doubling time as a function of channel width in channels of 15 (red circles) and 20 μm (black squares) length. All measurements have been done at 28°C. Data points represent the average of three independent measurements. In each measurement, more than 300 cell births have been analyzed. Error bars are s.e.m. of the three independent measurements. Blue horizontal lines represent measurements of cells from liquid culture tubes. The width of these lines corresponds to s.e.m. All other solid lines are linear fits to the data. (E) Logarithm of birth volume, V_b, as a function inverse of doubling time, $T_d^{-1}$. Solid line shows the fit of the data to the universal growth law, $V_b=\left(\frac{1}{2}\right)V_i•2^{\frac{C+D}{T_d}}$. In the universal growth law, V_i is cell volume at the initiation of replication, C the time taken to replicate DNA and D the time taken from the end of replication to cell division. Here V_i and C+D are treated as fitting parameters with best fit values $V_i=0.79\pm 0.08\mu m^3$ and C+D = 103±11 min. The errors are standard error.