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. 2018 May 1;9:412. doi: 10.3389/fphar.2018.00412

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Copyright © 2018 Zhang, Terrando, Xu, Bang, Jordt, Maixner, Serhan and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Perioperative DHA treatment increases RvD1 levels in serum and spinal cord. (A) RvD1 standard curve produced by the Cayman Chemical ELISA assay, demonstrating reliable measurements within the specified concentration range. RvD1 levels in serum samples (B), brain samples (C), and spinal cord samples (D) of naïve mice and mice after sham surgery or bone fracture with vehicle and DHA treatment (the same as described in Figure 1). Samples were collected at post-operative day 5 (POD 5). Note that RvD1 levels are elevated after sham surgery and in serum and spinal cord samples after the DHA treatment. ^∗P < 0.05, one-way ANOVA; n = 3–10 mice per group. Data are presented as mean ± SEM.