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. 2018 May 1;9:263. doi: 10.3389/fphar.2018.00263

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Copyright © 2018 Guan, Wang, Li, Ding, Li, Xu, Wang, Chen, Yang, Long, Cai, Zhang, Liang, Dong, Zhao, Zhang, Sun and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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STS inhibited CSE-induced production of inflammation cytokines in RAW 264.7 macrophages. RAW 264.7 macrophages were cultured with and without CSE and/or 1, 2.5, or 5 μM STS for 24 h. (A) The viabilities of macrophages treated as indicated were measured by CCK-8 assay. (B,C) The supernatant was collected to detect the content of TNF-α and IL-1β by ELISA. (D,E) The protein level of IL-1β in the macrophages was analyzed by Western blot. Data are presented as mean ± SEM, n = 3, ^∗P < 0.05; ^∗∗P < 0.01.