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. 2018 May 1;9:263. doi: 10.3389/fphar.2018.00263

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Copyright © 2018 Guan, Wang, Li, Ding, Li, Xu, Wang, Chen, Yang, Long, Cai, Zhang, Liang, Dong, Zhao, Zhang, Sun and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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STS inhibited CSE-induced oxidative stress and MMP-9 upregulation in macrophages. RAW 264.7 macrophages were treated with CSE and different concentrations of STS (1, 2.5, and 5 μM) for 24 h. (A) The intracellular ROS was determined using a fluorescence microplate according to the ROS assay kit. The relative protein levels of NOX1 (B), HO-1 (C), and MMP-9 (D) were determined by Western blot. The mRNA levels of HO-1 (E) and MMP-9 (F) in the lung tissues were analyzed by RT-PCR. Data are presented as mean ± SEM, n = 3, ^∗P < 0.05; ^∗∗P < 0.01.