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STS promoted CSE-induced HIF-1α protein degradation in macrophages. RAW 264.7 macrophages were treated with CSE for 24 h with or without 5 μM STS, followed by incubation with cycloheximide (CHX) from 0 to 30 min. (A) Cell lysates were then collected and prepared for Western blot analysis using antibodies against HIF-1α or β-actin. (B) The relative intensity of HIF-1α protein content was quantified. Data are presented as mean ± SEM, n = 3.
