Figure 5.

Co-localization of γH2AX and 53bp1 in retinal tissue of C3H and rd1 mice. Vertical frozen sections of developing retina of C3H and rd1 mice were stained with a cocktail of anti γH2AX and 53bp1 antibodies. Only INL and ONL are shown at high magnification. (A–D”) C3H mice at p13 and p28. (E–H”) rd1 mouse retina at p13 and p28. Numerous γH2AX immunoreactive foci are visible throughout the nuclear layers. 4–6 double labeled foci per field are visible at p13 in both mouse lines (arrows). At p28 only one or two double labeled foci per field are detectable (arrows). Co-localization of γH2AX immunoreactive foci and 53bp1 pan nuclear staining in the INL of C3H and rd1 mice at p13 are presented in Figure 5-1 of extended data. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. All scales = 20 μm.