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Global changes in SC ceramides with delipidation. (a,b) Composite fluorescent image of a BODIPY FL C12-Sphingomyelin ceramide stained circular SC sample (R = 3 mm) retaining a normal lipid composition (a) and following delipidation (b). Scale bars, 1 mm. (c) Average normalized lipid fluorescence within the inner R/2 region of control and delipidated SC. Error bars denote the mean ± s.d. of n = 3 individual SC samples for each lipid condition.
