Table 5.
Proteins associated with Non-Syndromic Hearing Loss segregating with caveolae in SL pericytes. The table shows proteins implicated in nonsyndromic hearing loss pathologies segregating with caveolae in treated and untreated cells. The highest number of unique peptides identifying the proteins is given in the table (n CTRL and n GTM) as well as their UniProt identifiers. The proteins myosin heavy chain 14 (MYH14), myosin heavy chain 9 (MYH9), Wolframin (WFS1), Lysyl-tRNA synthase (KARS) have been previously described in the SL. The proteins Diaphanous 1 (DIAPH1), MYH14, MYH9, unconventional myosin VI (MYO6), Radixin (RXD), TRIO and filamentous actin binding protein (TRIOBP), Taperin (TPRN), WFS1, KARS, Serpin B6 (SERPINB6), tight junction protein ZO-2 (Tjp2), polyribonucleotide-nucleotidyl transferase (PNP1), segregated with caveolae in both in untreated and GTM treated cells. One protein, Calcium integrin-binding family member 2 protein (CIB2), exclusively segregated with caveoale in untreated cells and two proteins Methionine Sulfoxide Reductase B3 (MSRB3) and Coiled Coil Domain Containing protein 50 (CCDC50) segregated exclusively in GTM treated cells
| Protein name | Gene | Function | n CTRL | n GTM | UniProt ID |
|---|---|---|---|---|---|
| Diaphanous 1 | DIAPH1 | Cytoskeleton and mobility | 4 | 5 | O08808 |
| Myosin Heavy Chain14 | MYH14 | Motor protein | 2 | 2 | Q6URW6 |
| Myosin Heavy Chain9 | MYH9 | Motor protein | 100 | 108 | Q8VDD5 |
| Unconventional myosin 6 | MYO6 | Motor protein | 6 | 11 | Q64331 |
| Radixin | RDX | Cytoskeleton | 6 | 10 | P26043 |
| TRIO filamentous actin binding protein | TRIOBP | Cytoskeleton | 12 | 12 | Q99KW3 |
| Taperin | TPRN | Cytoskeleton | 6 | 6 | A2AI08 |
| Wolframin | WFS1 | Regulation of Ca2+ homeostasis | 14 | 14 | P56695 |
| Lysyl-tRNA-synthase | KARS | Attach amino-acid to its cognate tRNA | 9 | 9 | Q99MN1 |
| Serpin B6 | SERPINB6 | Protease inhibitor | 10 | 11 | Q60854 |
| Tight junction protein ZO-2 | TJP2 | Role in tight and adherents junctions | 3 | 3 | Q9Z0U1 |
| Polyribonucleotide-nucleotidyl transferase | PNPT1 | Degrades mRNA | 2 | 1 | Q8K1R3 |
| Calcium integrin binding protein 2 | CIB2 | Calcium-binding regulatory protein | 1 | Q9Z309 | |
| Methionine R sulfoxide reductase | MSRB3 | Antioxidant | 2 | Q8BU85 | |
| Coiled coil domain containing protein | CCDC50 | Cytoskeleton | 2 | Q810U5 |