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. 2018 Apr 16;10(5):e8515. doi: 10.15252/emmm.201708515

Figure EV3. Lack of Patl2 does not affect number of oocytes produced, but impairs oocyte growth.

Figure EV3

  1. Representative images for a GV oocyte from WT and Patl2 −/− females stimulated with 5 UI PSMG. Scale bars = 35 μm.
  2. Comparison of WT and Patl2 −/− GV oocyte diameter. In the absence of Patl2, the diameter (mean ± SEM) dropped from 68.6 ± 0.5 to 64.5 ± 0.8 μm (P < 0.0001). Statistical difference was assessed using unpaired two‐tailed t‐test with Welch's correction.
  3. The number of oocytes (mean ± SEM) harvested 13 h after full hormonal stimulation (PMSG followed 48 h later by 5 UI HCG) was similar in WT and Patl2‐deficient females. Statistical difference was assessed using unpaired two‐tailed t‐test.
  4. Images of a MII oocytes from WT and Patl2 −/− females after stimulation (pb = 1st polar body). Scale bars = 35 μm.
  5. Diameter of WT and Patl2 −/− MII oocytes (mean ± SEM), the mean drops from 70.6 ± 0.3, n = 44 in the WT to 66.9 ± 0.3, n = 28 in Patl2 −/− (P < 0.0001), oocytes were collected from three females in each case. Statistical difference was assessed using unpaired two‐tailed t‐test.