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(Panel A) Chromatogram displaying the SIM of the dominant product formed when 1 mM MIA-t3NosM (black), MIA-t3NosMglu→ala (blue), and [γ-carboxy-18O]-MIA-t3NosM (red) are used in the NosN assay with 50 μM enzyme. (Panel B) MS/MS spectrum of the corresponding parent ion from panel A.
