Fig. 4. Enhanced expression of glycolytic genes in livers of DEN-Wwox^ΔHep mice.

a mRNA expression levels of HIF1α glycolytic target genes in DEN-treated control and Wwox^ΔHep (cKO) mice at the age of 1 a, 3 b, 6 c and 10 d months (n = 3 for each group). e Immunohistochemical staining of HIF1α, PKM2, and GLUT1 in 6 and 10 months of DEN-treated Wwox^ΔHep and control liver tissues. Images were taken at × 40 magnification (Bar = 100μm). f ChIP experiment with HIF1α antibody on DEN-treated control and Wwox^ΔHep (cKO) mice followed by qRT-PCR analysis of HIF1α target genes. * P value < 0.05, ** P value < 0.01, *** P value < 0.001. Error bars indicate ± SEM